remedy with sodium bisulfite deaminates unmethylated cytosine to uracil, While 5mC will not be afflicted. Subsequent PCR amplification will replace the uracils by thymines. Reads from NGS libraries produced in parallel https://emilypbrn795073.blogscribble.com/30076651/the-definitive-guide-to-dna-methylation-test-without-insurance